Neutralisation of biological activities of pertussis toxin with a monoclonal antibody

H. Anwar*, L. A.E. Ashworth, Simon Funnell, A. Robinson, L. I. Irons

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Three monoclonal antibodies (L5, L7 and L10) against pertussis toxin (PT) were produced by the fusion of myeloma cells with spleen cells of mice immunised with pertussis toxoid and PT. Immunoblotting showed that each monoclonal antibody bound to more than one subunit of the toxin. L5 and L7 bound predominantly to subunit S3 and with much less affinity to subunit S1, while L10 bound mainly to subunit S1. L7 strongly neutralised the lymphocytosis-promoting and histamine-sensitising activities of PT, whereas L5 and L10 showed no or only partial neutralising effect on these activities. The strongest neutralisation of PT activity in haemagglutination and Chinese hamster ovary (CHO) cell assays was also found with L7.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalFEMS Microbiology Letters
Volume44
Issue number1
DOIs
Publication statusPublished - Sep 1987

Bibliographical note

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Bordetella pertussis
  • Immunoblotting
  • Monoclonal antibody
  • Pertussis toxin

Fingerprint

Dive into the research topics of 'Neutralisation of biological activities of pertussis toxin with a monoclonal antibody'. Together they form a unique fingerprint.

Cite this