Nucleotide Sequence of the Gene Coding for Proteolytic (Group I) Clostridium botulinum Type F Neurotoxin: Genealogical Comparison with other Clostridial Neurotoxins

Michael Elmore, Roger A. Hutson, Matthew D. Collins, Nicola J. Bodsworth, Sarah M. Whelan, Nigel P. Minton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Botulinum neurotoxin type F can be produced by proteolytic or non-proreolytic strains of C. botulinum, or from C. baratii. In recent years, the sequences of all toxin types except that of proteolytic type F toxin have been determined. We have cloned the neurotoxin gene from proteolytic C. botulinum (type F Langeland) as a series of overlapping PCR fragments, and determined the nucleotide sequence. The translated sequence demonstrates a toxin of 1278 amino acid residues, which has highest similarity with non-proteolytic type F toxin. Phylogenetic trees based on toxin sequences show that the type F sequences fall into a naturally distinct group, although the strains producing these toxins are not phylogenetically closely related.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalSystematic and Applied Microbiology
Volume18
Issue number1
DOIs
Publication statusPublished - 1995

Bibliographical note

Funding Information:
Acknowledgements. CAMR wishes to acknowledge the financial support of the UK Department of Health and Ministry of Defence (grant 2238/002), and the US Army Medical Research Development Command (grant DAMDI7-90-20033).

Keywords

  • Clostridium botulinum
  • Gene sequence
  • Neurotoxin

Fingerprint

Dive into the research topics of 'Nucleotide Sequence of the Gene Coding for Proteolytic (Group I) Clostridium botulinum Type F Neurotoxin: Genealogical Comparison with other Clostridial Neurotoxins'. Together they form a unique fingerprint.

Cite this