Positive predictive value of the Becton Dickinson VIPER system and the ProbeTec GC Qx assay, in extracted mode, for detection of Neisseria gonorrhoeae

Cassie F. Pope, Phillip Hay, Sarah Alexander, Kay Capaldi, Jayshree Dave, S. Tariq Sadiq, Catherine Ison, Timothy Planche

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Objectives: Performance of the new Becton Dickinson ProbeTec GC Q x assay on the BD VIPER platform was evaluated to ascertain whether confirmatory testing is required in our clinical setting. Methods: Positive predictive value (PPV) was determined by comparison with culture and a confirmatory nucleic acid amplification test (NAAT)-based Neisseria gonorrhoeae assay from genital and extragenital samples (rectal and pharyngeal) collected from a genitourinary medicine (GUM) clinic. Results: Among 14 223 clinical genital samples, 149 (1.0%) specimens were positive using the ProbeTec GC Q x assay, automated on the VIPER platform; 141 of these were confirmed by either culture or a real-time PCR targeting two gonococcal-specific targets (PPV 94.6%; 95% CI 90% to 98%). Among 840 pharyngeal samples, 26 (3.1%) were positive by the ProbeTec GC Qx assay; 13 were confirmed (PPV 50%; 95% CI 30% to 70%). Among 593 rectal samples, 17 tested positive by the ProbeTec GC Qx assay; all were confirmed (PPV 100%; 95% CI 80% to 100%). Conclusions: The lower 95% CI of the PPV for the ProbeTec GC Qx assay for genital specimens was >90% in this GUM clinic population, and therefore confirmatory testing for genital specimens is not required. Confirmatory testing of pharyngeal and rectal samples should continue in line with national guidelines.

Original languageEnglish
Pages (from-to)465-469
Number of pages5
JournalSexually Transmitted Infections
Volume86
Issue number6
DOIs
Publication statusPublished - Nov 2010

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