Prevalence of porA pseudogene deletion among Neisseria gonorrhoeae isolates referred to the UK's Gonococcal Resistance to Antimicrobials Surveillance Program

Martina Toby, Pamela Saunders, Michelle Cole, Vlad Grigorjev, Sarah Alexander*, Catherine Ison

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

porA pseudogene-negative Neisseria gonorrhoeae isolates produce false-negative results when examined by polymerase chain reaction (PCR) with porA pseudogene targets. In the present study, 533 representative gonococcal isolates received in 2011 via the Gonococcal Resistance to Antimicrobials Surveillance Program were examined to determine the prevalence of porA-negative isolates. Less than 0.4% (2/533) of isolates were found to be reproducibly negative with the porA real-time PCR but were confirmed as N. gonorrhoeae with molecular, biochemical and immunological confirmatory tests. Sequencing revealed both isolates contained the Neisseria meningitidis porA gene. Low prevalence indicates that although these isolates do not present a major public health problem, microbiologists should remain vigilant.

Original languageEnglish
Pages (from-to)392-393
Number of pages2
JournalSexual Health
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Dive into the research topics of 'Prevalence of porA pseudogene deletion among Neisseria gonorrhoeae isolates referred to the UK's Gonococcal Resistance to Antimicrobials Surveillance Program'. Together they form a unique fingerprint.

Cite this