Protective Effect of Oral BCG and Inactivated Mycobacterium bovis Vaccines in European Badgers (Meles meles) Experimentally Infected With M. bovis

Ana Balseiro*, José Miguel Prieto, Vega Álvarez, Sandrine Lesellier, Dipesh Davé, Francisco J. Salguero, Iker A. Sevilla, José Antonio Infantes-Lorenzo, Joseba M. Garrido, Hans Adriaensen, Ramón A. Juste, Marta Barral

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

In Europe, badgers (Meles meles) are recognized as major tuberculosis (TB) reservoir hosts with the potential to transmit infection to associated cattle herds. Recent studies in Spain have demonstrated that vaccination with a heat-inactivated Mycobacterium bovis vaccine (HIMB) successfully protects captive wild boar and red deer against progressive disease. The aim of this study was to evaluate the efficacy of two oral vaccines against TB in a badger model: the live-attenuated M. bovis bacillus Calmette-Guérin BCG vaccine (Danish strain) and a HIMB vaccine. Twenty-four badgers were separated in three treatment groups: oral vaccinated with live BCG (108 CFU, n = 5), oral vaccinated with HIMB (107 CFU, n = 7), and unvaccinated controls (n = 12). All badgers were experimentally infected with M. bovis (103 CFU) by the endobronchial route targeting the right middle lung lobe. Throughout the study, clinical, immunological, pathological, and bacteriological parameters of infection were measured. Both vaccines conferred protection against experimental TB in badger, as measured by a reduction of the severity and lesion volumes. Based on these data, HIMB vaccination appears to be a promising TB oral vaccine candidate for badgers in endemic countries.

Original languageEnglish
Article number41
JournalFrontiers in Veterinary Science
Volume7
DOIs
Publication statusPublished - 4 Feb 2020

Bibliographical note

Funding Information:
This study was funded by a grant from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), reference number RTA2014-00002-C02 (FEDER co-funded), the Principado de Asturias, PCTI 2018–2020 (GRUPIN: IDI2018-000237 and FEDER), and Departamento de Desarrollo Económico e Infraestructuras del Gobierno Vasco. This study was also funded by Ministerio de Ciencia, Innovación y Universidades (MCIU), the Agencia Estatal de Investigación (AEI) reference project RTI2018-096010-B-C21 and Fondo Europeo de Desarrollo Regional (FEDER). DEFRA also funded APHA staff for their participation in the study and for the purchase of BCG from Statens Serum Institute (Copenhagen, Denmark).

Funding Information:
Authors thank Cristina Blanco V?zquez, Miriam Mart?nez de Egidua, Xeider Gerrikagoitia, Valentin Wacheux, Amaia Etxezarreta, Olalla Torrontegui, Si Palmer, Bernat P?rez de Val, and Enric Vidal for their collaboration during the processing of samples, Gareth Williams for providing BCG and Colin Birch for helping in calculating the disease burden scores. Authors also thank Kevin P. Dalton for critically reviewing the manuscript. Funding. This study was funded by a grant from Instituto Nacional de Investigaci?n y Tecnolog?a Agraria y Alimentaria (INIA), reference number RTA2014-00002-C02 (FEDER co-funded), the Principado de Asturias, PCTI 2018?2020 (GRUPIN: IDI2018-000237 and FEDER), and Departamento de Desarrollo Econ?mico e Infraestructuras del Gobierno Vasco. This study was also funded by Ministerio de Ciencia, Innovaci?n y Universidades (MCIU), the Agencia Estatal de Investigaci?n (AEI) reference project RTI2018-096010-B-C21 and Fondo Europeo de Desarrollo Regional (FEDER). DEFRA also funded APHA staff for their participation in the study and for the purchase of BCG from Statens Serum Institute (Copenhagen, Denmark).

Publisher Copyright:
© Copyright © 2020 Balseiro, Prieto, Álvarez, Lesellier, Davé, Salguero, Sevilla, Infantes-Lorenzo, Garrido, Adriaensen, Juste and Barral.

Keywords

  • BCG vaccine
  • Mycobacterium bovis heat-inactivated (HIMB) vaccine
  • badger
  • efficacy
  • tuberculosis

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