Proteomic analysis of Neisseria lactamica and N eisseria meningitidis outer membrane vesicle vaccine antigens

Thomas E. Vaughan, Paul J. Skipp, C. David O'Connor, Michael Hudson, Richard Vipond, Michael J. Elmore, Andrew Gorringe

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    46 Citations (Scopus)

    Abstract

    Vaccines to prevent meningococcal disease have been developed from the outer membrane vesicles (OMVs) of Neisseria meningitidis and the related commensal organism Neisseria lactamica. In addition to lipopolysaccharide and the major porins, these vaccines contain a large number of proteins that are incompletely characterised. Here we describe comparative proteomic analyses of the N. lactamica OMV vaccine and OMVs from a serogroup B strain of N. meningitidis. Tandem mass-spectrometry data for trypsinised N. lactamica OMV vaccine were matched to an incompletely assembled genome sequence from the same strain to give 65 robust protein identifications and a further 122 single- or two-peptide matches. Fifty-seven N. meningitidis K454 proteins were identified robustly (and a further 68 from single- or two-peptide matches) by inference from the N. meningitidis MC58 genome. The results suggest that OMVs have a hitherto unappreciated complexity and pinpoint novel candidate antigens for further characterisation.

    Original languageEnglish
    Pages (from-to)5277-5293
    Number of pages17
    JournalVaccine
    Volume24
    Issue number25
    DOIs
    Publication statusPublished - 19 Jun 2006

    Keywords

    • Neisseria lactamica
    • Neisseria meningitidis
    • Outer membrane vesicle
    • Proteomics
    • Tandem mass spectrometry
    • Vaccine

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