Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis

Andrew Borman, Christopher Linton, Debra Oliver, Michael Palmer, Adrien Szekely, Frank C. Odds, Elizabeth Johnson

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

Original languageEnglish
Pages (from-to)2307-2310
Number of pages4
JournalJournal of Clinical Microbiology
Volume47
Issue number7
DOIs
Publication statusPublished - Jul 2009

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