Radiation induction of micronuclei in human lymphocytes

J. S. Prosser*, Jayne Moquet, D. C. Lloyd, Alan Edwards

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

152 Citations (Scopus)

Abstract

An examination of the cytokinesis-blocked micronucleus technique confirmed its potential usefulness as a method of biological dosimetry for radiation accidents. Several advantages of the system are discussed. It has been demonstrated that under the conditions of these experiments, the blocking agent, cytochalasin B does not induce micronuclei or unstable chromosome aberrations. The induction of sister-chromatid exchanges proved just significant. Analysis of the dose response for 250 kVp X-rays indicates that although the Y = αD + βD2 model fits the data, the relationship does not correspond to that for total aberration induction as might have been expected. The background frequency of micronuclei and the value of the α coefficient are higher than for total aberrations and the β term is lower. This indicates that simple incorporation of acentric chromosome fragments into micronuclei may not wholly account for the phenomenon.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume199
Issue number1
DOIs
Publication statusPublished - May 1988

Keywords

  • Cytochalasin B
  • Human lymphocytes
  • Micronucleus
  • Radiation dosimetry

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