RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for screening multiple colonies

Ka Ning To, Oliver Powell, Dorota Jamrozy, Rachel Kopunova, Kyriaki Anastasiadou, Amadou Faal, Ousman Secka, Victoria Chalker, Kirsty Le Doare, Elita Jauneikaite*

*Corresponding author for this work

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Abstract

Group B Streptococcus (GBS) is a leading cause of neonatal meningitis, pneumonia, and sepsis. The biggest contributing factor of neonatal infections is due to vertical transmission from maternal colonisation of GBS in the genitourinary tract. Multiple serotype colonisation is often not investigated in epidemiological studies, but it is an important consideration for serotype-based vaccine development and implementation to ensure less abundant serotypes are not under-represented. In this study, we show that RAPD PCR is a quick tool useful in screening the presence of genetically different strains using multiple colony picks from a single patient swab. We observed a maximum of five different GBS strains colonising a single patient at a specific time.

Original languageEnglish
Article number106322
JournalJournal of Microbiological Methods
Volume190
Early online date8 Sep 2021
DOIs
Publication statusE-pub ahead of print - 8 Sep 2021

Bibliographical note

Funding Information: We thank the Imperial BRC Genomics Facility who provided resources and support that have contributed to the research results reported within this paper. The Imperial BRC Genomics Facility is supported by NIHR funding to the Imperial Biomedical Research Centre . EJ is affiliated with the National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Healthcare Associated Infections and Antimicrobial Resistance at Imperial College London in partnership with Public Health England (PHE) in collaboration with, Imperial Healthcare Partners, University of Cambridge, and University of Warwick.

This work was supported by The Wellcome Trust [ 104482/Z/14/Z ], The Thrasher Research Fund [ 15055 ], The Bill & Melinda Gates Foundation [ OPP1153630 ] and The Rosetrees Trust & The Stoneygate Trust [ M683 ]. The funders had no role in study design, data collection, analysis, decision to publish, or preparation of the manuscript.

Open Access: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Publisher Copyright: Crown Copyright © 2021 Published by Elsevier B.V.

Citation: Ka-Ning To, Oliver Powell, Dorota Jamrozy, Rachel Kopunova, Kyriaki Anastasiadou, Amadou Faal, Ousman Secka, Victoria Chalker, Kirsty Le Doare, Elita Jauneikaite, RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for screening multiple colonies, Journal of Microbiological Methods, Volume 190, 2021, 106322, ISSN 0167-7012,

DOI: https://doi.org/10.1016/j.mimet.2021.106322.

Keywords

  • Co-colonisation
  • Genetic diversity
  • Group B Streptococcus
  • Multiple serotypes
  • RAPD PCR
  • S. agalactiae
  • Screening

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