Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp.

Monia Vitullo, Kathie Ann Grant, Michela Lucia Sammarco, Manuela Tamburro, Giancarlo Ripabelli, Corinne Amar

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

A 5′-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L. monocytogenes.

Original languageEnglish
Pages (from-to)68-70
Number of pages3
JournalMolecular and Cellular Probes
Volume27
Issue number1
DOIs
Publication statusPublished - Feb 2013

Keywords

  • Listeria monocytogenes
  • Listeria spp.
  • Molecular epidemiology
  • Real-time PCR
  • Serogrouping

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