Retrieval of the complete coding sequence of the UK-endemic tatenale orthohantavirus reveals extensive strain variation and supports its classification as a novel species

Joseph G. Chappell, Theocharis Tsoleridis, Okechukwu Onianwa, Gabby Drake, Ian Ashpole, Phillipa Dobbs, William Edema, Frederick Kumi-Ansah, Malcolm Bennett, Rachael E. Tarlinton, Jonathan K. Ball*, C. Patrick McClure

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (Microtus agrestis) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus.

Original languageEnglish
Article number454
JournalViruses
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 2020
Externally publishedYes

Bibliographical note

Funding Information:
Funding: This research was funded by the Medical Research Council studentship award, grant number 1651320.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Field vole
  • Hantavirus
  • High-throughput sequencing
  • Orthohantavirus
  • United Kingdom
  • Virus discovery

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