Rhizobium leguminosarum NodT is related to a family of outer-membrane transport proteins that includes TolC, PrtF, CyaE and AprF

Rafael Rivilla, J. Mark Sutton, J. Allan Downie*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Cells containing a protein fusion consisting of the Rhizobium leguminosarum bv. viciae nodulation protein, NodT, fused to PhoA, produced alkaline phosphatase activity, indicating that the N terminus of NodT could translocate PhoA across the inner membrane. Cellular fractionation suggested that the NodT::PhoA fusion is targetted to the outer membrane. NodT resembles a family of bacterial outer membrane proteins including TolC, PrtF, CyaE and AprF, which are involved in secretion. By analogy, NodT (together with the inner membrane putative transport proteins Nodl and NodJ) is proposed to be involved in the secretion of nodulation factors.

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalGene
Volume161
Issue number1
DOIs
Publication statusPublished - 1995
Externally publishedYes

Bibliographical note

Funding Information:
receiveda postdoctorafle llowshipfrom the Spanish Ministry for Sciencea nd Educationa nd the work was supportebdy a grant-in-aifdr omthe AFRC and AFRC-PMB grant PG208/548.

Keywords

  • Legume
  • lipo-oligosaccharide
  • nod
  • nodulation factor
  • nodule

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