Cells containing a protein fusion consisting of the Rhizobium leguminosarum bv. viciae nodulation protein, NodT, fused to PhoA, produced alkaline phosphatase activity, indicating that the N terminus of NodT could translocate PhoA across the inner membrane. Cellular fractionation suggested that the NodT::PhoA fusion is targetted to the outer membrane. NodT resembles a family of bacterial outer membrane proteins including TolC, PrtF, CyaE and AprF, which are involved in secretion. By analogy, NodT (together with the inner membrane putative transport proteins Nodl and NodJ) is proposed to be involved in the secretion of nodulation factors.
Bibliographical noteFunding Information:
receiveda postdoctorafle llowshipfrom the Spanish Ministry for Sciencea nd Educationa nd the work was supportebdy a grant-in-aifdr omthe AFRC and AFRC-PMB grant PG208/548.
- nodulation factor