Robust Prediction of Resistance to Trimethoprim in Staphylococcus aureus

Philip W. Fowler, Kevin Cole, N. Claire Gordon, Angela Kearns, Martin J. Llewelyn, Tim E.A. Peto, Derrick W. Crook, A. Sarah Walker

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The rise of antibiotic resistance threatens modern medicine; to combat it new diagnostic methods are required. Sequencing the whole genome of a pathogen offers the potential to accurately determine which antibiotics will be effective to treat a patient. A key limitation of this approach is that it cannot classify rare or previously unseen mutations. Here we demonstrate that alchemical free energy methods, a well-established class of methods from computational chemistry, can successfully predict whether mutations in Staphylococcus aureus dihydrofolate reductase confer resistance to trimethoprim. We also show that the method is quantitatively accurate by calculating how much the most common resistance-conferring mutation, F99Y, reduces the binding free energy of trimethoprim and comparing predicted and experimentally measured minimum inhibitory concentrations for seven different mutations. Finally, by considering up to 32 free energy calculations for each mutation, we estimate its specificity and sensitivity. Fowler et al. demonstrate how alchemical free energy calculations not only can classify whether mutations in Staphylococcus aureus dihydrofolate reductase confer resistance to trimethoprim, an antibiotic, or not, but also that the method is quantitatively accurate.

Original languageEnglish
Pages (from-to)339-349.e4
JournalCell Chemical Biology
Volume25
Issue number3
DOIs
Publication statusPublished - 15 Mar 2018

Keywords

  • antibiotic susceptibility testing
  • antimicrobial resistance
  • clinical microbiology
  • free energy calculations
  • molecular dynamics

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