Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid

Javier Periz, Cristina Celma, Bo Jing, Justin N.M. Pinkney, Polly Roy, Achillefs N. Kapanidis

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Rotaviruses are the single most common cause of fatal and severe childhood diarrheal illness worldwide (>125 million cases annually). Rotavirus shares structural and functional features with many viruses, such as the presence of segmented double-stranded RNA genomes selectively and tightly packed with a conserved number of transcription complexes in icosahedral capsids. Nascent transcripts exit the capsid through 12 channels, but it is unknown whether these channels specialize in specific transcripts or simply act as general exit conduits; a detailed description of this process is needed for understanding viral replication and genomic organization. To this end, we developed a single molecule assay for capturing and identifying transcripts extruded from transcriptionally active viral particles. Our findings support a model in which each channel specializes in extruding transcripts of a specific segment that in turn is linked to a single transcription complex. Our approach can be extended to study other viruses and transcription systems.

Original languageEnglish
Pages (from-to)12042-12047
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number29
DOIs
Publication statusPublished - 16 Jul 2013
Externally publishedYes

Keywords

  • Channel Specialization
  • Reoviridae
  • Single Molecule Hybridization
  • Single-Stranded Rna

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