There is a growing need for rapid diagnostic methods to support stewardship of antibiotics. We describe an analytical platform for sample concentration to detect antimicrobial resistance (AMR) genes directly from human urine for the diagnosis of urinary tract infections (UTIs) that are resistant to antibiotics. A sample-processing unit concentrates plasmid DNA directly from urine using magnetic beads, followed by isothermal amplification of target genes. The sample preconcentration unit interfaces with a digital microfluidic platform (DMF) and scales the sample volume by 500-fold, preconcentrating DNA from 1 mL into a 2 μL droplet for downstream processing. Tests with a clinical strain of Klebsiella pneumoniae (NCTC 13443), spiked into human urine demonstrated a limit of detection of 104 cfu mL-1 and a "sample to answer" detection in approximately 30 minutes.