Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine

Sumit Kalsi, Martha Valiadi, Carrie Turner, Mark Sutton, Hywel Morgan

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

There is a growing need for rapid diagnostic methods to support stewardship of antibiotics. We describe an analytical platform for sample concentration to detect antimicrobial resistance (AMR) genes directly from human urine for the diagnosis of urinary tract infections (UTIs) that are resistant to antibiotics. A sample-processing unit concentrates plasmid DNA directly from urine using magnetic beads, followed by isothermal amplification of target genes. The sample preconcentration unit interfaces with a digital microfluidic platform (DMF) and scales the sample volume by 500-fold, preconcentrating DNA from 1 mL into a 2 μL droplet for downstream processing. Tests with a clinical strain of Klebsiella pneumoniae (NCTC 13443), spiked into human urine demonstrated a limit of detection of 104 cfu mL-1 and a "sample to answer" detection in approximately 30 minutes.

Original languageEnglish
Pages (from-to)168-177
Number of pages10
JournalLab on a Chip
Volume19
Issue number1
DOIs
Publication statusPublished - 7 Jan 2019

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