Isolated brush borders become labelled within 7.5 hours following injection of Na235SO4 into rabbits. The label is present in glycoprotein (73%) and in an hydrophobic glycolipid fraction (24%). T.l.c. and DEAE-cellulose chromatography indicate the presence of one principal sulphated glycolipid in which the sugars are glucose, galactose and glucosamine (1:2:2). Sialic acid residues are absent from the glycoproteins and are found only in minor ganglioside components. Ester sulphate is considered to contribute significantly to the anionic character of the membrane.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 28 Jul 1978|