35S-sulphate labelling of glycoproteins and glycolipids in rabbit small intestinal brush borders

John Cooper, P. W. Kent

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    Abstract

    Isolated brush borders become labelled within 7.5 hours following injection of Na235SO4 into rabbits. The label is present in glycoprotein (73%) and in an hydrophobic glycolipid fraction (24%). T.l.c. and DEAE-cellulose chromatography indicate the presence of one principal sulphated glycolipid in which the sugars are glucose, galactose and glucosamine (1:2:2). Sialic acid residues are absent from the glycoproteins and are found only in minor ganglioside components. Ester sulphate is considered to contribute significantly to the anionic character of the membrane.

    Original languageEnglish
    Pages (from-to)622-627
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume83
    Issue number2
    DOIs
    Publication statusPublished - 28 Jul 1978

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