The differences in short- and long-term varicella-zoster virus (VZV) immunoglobulin G levels following varicella vaccination of healthcare workers measured by VZV fluorescent-antibody-to-membrane-antigen assay (FAMA), VZV time-resolved fluorescence immunoassay and a VZV purified glycoprotein enzyme immunoassay

P. A.C. Maple, J. Haedicke, M. Quinlivan, S. P. Steinberg, A. A. Gershon, Kevin Brown, J. Breuer

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Healthcare workers (HCWs) reporting no history of varicella frequently receive varicella vaccination (vOka) if they test varicella-zoster virus (VZV) immunoglobulin G (IgG) negative. In this study, the utilities of VZV-IgG time-resolved fluorescence immunoassay (VZV-TRFIA) and a commercial VZV-IgG purified glycoprotein enzyme immunoassay (gpEIA) currently used in England for confirming VZV immunity have been compared to the fluorescent-antibody-to-membrane-antigen assay (FAMA). A total of 110 HCWs received two doses of vOka vaccine spaced 6 weeks apart and sera collected pre-vaccination (n = 100), at 6 weeks post-completion of vaccination (n = 86) and at 12-18 months follow-up (n = 73) were analysed. Pre-vaccination, by FAMA, 61 0% sera were VZV IgG negative, and compared to FAMA the sensitivities of VZV-TRFIA and gpEIA were 74 4% [95% confidence interval (CI) 57 9-87 0] and 46 2% (95% CI 30 1-62 8), respectively. Post-completion of vaccination the seroconversion rate by FAMA was 93 7% compared to rates of 95 8% and 70 8% determined by VZV-TRFIA and gpEIA, respectively. At 12-18 months follow-up seropositivity rates by FAMA, VZV-TRFIA and gpEIA were 78 1%, 74 0% and 47 9%, respectively. Compared to FAMA the sensitivities of VZV-TRFIA and gpEIA for measuring VZV IgG following vaccination were 96 4% (95% CI 91 7-98 8) and 74 6% (95% CI 66 5-81 6), respectively. Using both FAMA and VZV-TRFIA to identify healthy adult VZV susceptibles and measure seroconversion showed that vOka vaccination of HCWs is highly immunogenic.

Original languageEnglish
Pages (from-to)2345-2353
Number of pages9
JournalEpidemiology and Infection
Volume144
Issue number11
DOIs
Publication statusPublished - 1 Aug 2016

Keywords

  • Fluorescent-antibody-to-membrane-antigen-assay
  • VZV glycoprotein EIA
  • VZV immunoglobulin G
  • VZV time-resolved fluorescence immunoassay
  • healthcare workers
  • vOka vaccine

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