The exclusion of dead bacterial cells is essential for accurate molecular analysis of clinical samples

G. B. Rogers, P. Marsh, A. F. Stressmann, Collette Allen, T. V.W. Daniels, M. P. Carroll, K. D. Bruce

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

The DNA-based techniques used to detect bacteria in clinical samples are unable to discriminate between live bacteria, dead bacteria, and extracellular DNA. This failure to limit analysis to viable bacterial cells represents a significant problem, leading to false-positive results, as well as a failure to resolve the impact of antimicrobial therapy. The use of propidium monoazide treatment significantly reduces the contribution of dead cells and extracellular DNA to such culture-independent analyses. Here, the increased ability to resolve the impact of antibiotic therapy on Pseudomonas aeruginosa load in cystic fibrosis respiratory samples reveals statistically significant changes that would otherwise go undetected.

Original languageEnglish
Pages (from-to)1656-1658
Number of pages3
JournalClinical Microbiology and Infection
Volume16
Issue number11
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Keywords

  • Antibiotic treatment
  • Bacterial viability
  • Cystic fibrosis
  • Propidium monoazide
  • Q-PCR

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