The Genetic Diversity of Rickettsiella Symbionts in Ixodes ricinus Throughout Europe

Aitor Garcia-Vozmediano*, Laura Tomassone, Manoj Fonville, Luigi Bertolotti, Dieter Heylen, Nannet D. Fabri, Jolyon M. Medlock, Ard M. Nijhof, Kayleigh M. Hansford, Hein Sprong, Aleksandra I. Krawczyk

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Rickettsiella species are bacterial symbionts that are present in a great variety of arthropod species, including ixodid ticks. However, little is known about their genetic diversity and distribution in Ixodes ricinus, as well as their relationship with other tick-associated bacteria. In this study, we investigated the occurrence and the genetic diversity of Rickettsiella spp. in I. ricinus throughout Europe and evaluated any preferential and antagonistic associations with Candidatus Midichloria mitochondrii and the pathogens Borrelia burgdorferi sensu lato and Borrelia miyamotoi. Rickettsiella spp. were detected in most I. ricinus populations investigated, encompassing a wide array of climate types and environments. The infection prevalence significantly differed between geographic locations and was significantly higher in adults than in immature life stages. Phylogenetic investigations and protein characterization disclosed four Rickettsiella clades (I–IV). Close phylogenetic relations were observed between Rickettsiella strains of I. ricinus and other arthropod species. Isolation patterns were detected for Clades II and IV, which were restricted to specific geographic areas. Lastly, although coinfections occurred, we did not detect significant associations between Rickettsiella spp. and the other tick-associated bacteria investigated. Our results suggest that Rickettsiella spp. are a genetically and biologically diverse facultative symbiont of I. ricinus and that their distribution among tick populations could be influenced by environmental components.

Original languageEnglish
JournalMicrobial Ecology
DOIs
Publication statusAccepted/In press - 2021

Bibliographical note

Funding Information:
This research was financially supported by the Dutch Ministry of Health, Welfare and Sport (VWS) and a grant from the European Interreg North Sea Region program, as part of the NorthTick project. AMN received financial support from the Federal Ministry of Education and Research (BMBF) under project number 01KI1720 as part of the ‘Research Network Zoonotic Infectious Diseases’. DH is funded by the Marie Sklodowska-Curie Actions (EU-Horizon 2020, Individual Global Fellowship, project no 799609).

Publisher Copyright:
© 2021, The Author(s).

Keywords

  • Facultative symbionts
  • Ixodes ricinus ecology
  • Rickettsiella
  • Tick-borne bacteria co-infection
  • Tick-borne pathogens

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