The Murray collection of pre-antibiotic era Enterobacteriacae: A unique research resource

Kate S. Baker, Edward Burnett, Hannah McGregor, Ana Deheer-Graham, Christine Boinett, Gemma C. Langridge, Alexander M. Wailan, Amy K. Cain, Nicholas R. Thomson, Julie Russell, Julian Parkhill*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance.

Original languageEnglish
Article number97
JournalGenome Medicine
Volume7
Issue number1
DOIs
Publication statusPublished - 28 Sep 2015

Bibliographical note

Funding Information:
The authors thank David Harris and the WTSI sequencing teams for coordination of sample sequencing, and Philippa Bracegirdle and Steven Brimble for assistance in designing initial work flows at PHE. WTSI authors were funded by grant number 980561. CB and AC are supported by MRC grant G1100100/1. KSB is in receipt of a Wellcome Trust Postdoctoral Training Fellowship for Clinicians (106690/Z/14/Z). The authors are also grateful to Vicki Hughes, Naomi Datta, Tegid Matthews, Peter Sneath, Laurence Rowland Hill, Rita Legros, and R.E.G. Murray who coordinated the transfer of the strains to the NCTC and performed the laboratory analysis of plasmid content.

Publisher Copyright:
© 2015 Baker et al.

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