The use of next generation sequencing for improving food safety: Translation into practice

Balamurugan Jagadeesan, Peter Gerner-Smidt, Marc W. Allard, Sébastien Leuillet, Anett Winkler, Yinghua Xiao, Samuel Chaffron, Jos Van Der Vossen, Silin Tang, Mitsuru Katase, Peter McClure, Bon Kimura, Lay Ching Chai, John Chapman, Kathleen Grant

Research output: Contribution to journalReview articlepeer-review

62 Citations (Scopus)

Abstract

Next Generation Sequencing (NGS) combined with powerful bioinformatic approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of single isolates allows the most detailed comparison possible hitherto of individual strains. The two principle approaches for strain discrimination, single nucleotide polymorphism (SNP) analysis and genomic multi-locus sequence typing (MLST) are showing concordant results for phylogenetic clustering and are complementary to each other. Metabarcoding and metagenomics, applied to total DNA isolated from either food materials or the production environment, allows the identification of complete microbial populations. Metagenomics identifies the entire gene content and when coupled to transcriptomics or proteomics, allows the identification of functional capacity and biochemical activity of microbial populations. The focus of this review is on the recent use and future potential of NGS in food microbiology and on current challenges. Guidance is provided for new users, such as public health departments and the food industry, on the implementation of NGS and how to critically interpret results and place them in a broader context. The review aims to promote the broader application of NGS technologies within the food industry as well as highlight knowledge gaps and novel applications of NGS with the aim of driving future research and increasing food safety outputs from its wider use.

Original languageEnglish
Pages (from-to)96-115
Number of pages20
JournalFood Microbiology
Volume79
DOIs
Publication statusPublished - Jun 2019

Keywords

  • Data sharing
  • Food safety and quality
  • Implementation
  • Metabarcoding
  • Metagenomics
  • Microbiology
  • Next generation sequencing
  • Whole genome sequencing

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