Verification by the FISH translocation assay of historic doses to Mayak workers from external gamma radiation

Natalia V. Sotnik*, Tamara V. Azizova, Firouz Darroudi, Elizabeth Ainsbury, Jayne Moquet, Janna Fomina, David C. Lloyd, Pat A. Hone, Alan Edwards

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The aim of this study was to apply the fluorescence in situ hybridization (FISH) translocation assay in combination with chromosome painting of peripheral blood lymphocytes for retrospective biological dosimetry of Mayak nuclear power plant workers exposed chronically to external gamma radiation. These data were compared with physical dose estimates based on monitoring with badge dosimeters throughout each person’s working life. Chromosome translocation yields for 94 workers of the Mayak production association were measured in three laboratories: Southern Urals Biophysics Institute, Leiden University Medical Center and the former Health Protection Agency of the UK (hereinafter Public Health England). The results of the study demonstrated that the FISH-based translocation assay in workers with prolonged (chronic) occupational gamma-ray exposure was a reliable biological dosimeter even many years after radiation exposure. Cytogenetic estimates of red bone marrow doses from external gamma rays were reasonably consistent with dose measurements based on film badge readings successfully validated in dosimetry system “Doses-2005” by FISH, within the bounds of the associated uncertainties.

Original languageEnglish
Pages (from-to)445-451
Number of pages7
JournalRadiation and Environmental Biophysics
Volume54
Issue number4
DOIs
Publication statusPublished - 30 Aug 2015

Bibliographical note

Funding Information:
In memoriam of two outstanding scientists: Alan A. Edwards for his significant professional contribution to investigations of biological dosimetry of external radiation and Pat A. Hone for her significant efforts in completing the FISH analyses for this study. The study was financially supported by the European Union (Sixth Framework Program), Southern Urals Radiation Risk Research (SOUL) and the Federal Medical Biological Agency of Russia under Grant Agreement FIP6R-516478.

Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.

Keywords

  • Badge doses
  • Dose validation
  • External gamma rays
  • FISH
  • Translocations

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