Virulence in the chick model and stress tolerance of Salmonella enterica serovar Orion var. 15+

Roberto M. La Ragione, Karen E. Coles, Frieda Jorgensen, Tom J. Humphrey, Martin J. Woodward

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    7 Citations (Scopus)

    Abstract

    Three Salmonella enterica serovar Orion var. 15+ isolates of distinct provenance were tested for survival in various stress assays. All were less able to survive desiccation than a virulent S. Enteritidis strain, with levels of survival similar to a rpoS mutant of the S. Enteritidis strain, whereas one isolate (F3720) was significantly more acid tolerant. The S. Orion var. 15+ isolates were motile by flagellae and elaborated type-1 and curli-like fimbriae; surface organelles that are considered virulence determinants in Salmonella pathogenesis. Each adhered and invaded HEp-2 tissue culture cells with similar proficiency to the S. Enteritidis control but were significantly less virulent than S. Enteritidis in the one-day-old and seven-day-old chick model. Given an oral dose of 1 × 103 cfu to one-day-old chicken, S. Orion var. 15+ isolates colonised 25% of liver and spleens examined at 24 h whereas S. Enteritidis colonised 100% of organs by the same with the same dose. Given an oral dose of 1 × 107 cfu at seven-day old, S. Orion var. 15+ failed to colonise livers and spleens in any bird examined at 24 h whereas S. Enteritidis colonised 50% of organs by the same with the same dose. Based on the number of internal organs colonised, one of the three S. Orion var. 15+ isolates tested (strain F3720) was significantly more invasive than the other two (B1 and B7). Also, strain F3720 was shed less than either B1 or B7 supporting the concept that there may be an inverse relationship between the ability to colonise deep tissues and to persist in the gut. These data are discussed in the light that S. Orion var. 15+ is associated with sporadic outbreaks of human infection rather than epidemics.

    Original languageEnglish
    Pages (from-to)707-718
    Number of pages12
    JournalInternational Journal of Medical Microbiology
    Volume290
    Issue number8
    DOIs
    Publication statusPublished - 2001

    Bibliographical note

    Funding Information:
    Acknowledgements. The authors gratefully acknowledge the expert advice of Robin Sayers (VLA, Weybridge) on the statistical analysis of the data. This work was supported by project DH251 from the Department of Health, UK. M.J. Woodward is supported by Ministry of Agriculture Fisheries and Food, UK.

    Copyright:
    Copyright 2018 Elsevier B.V., All rights reserved.

    Keywords

    • Chicken infection
    • Environmental survival
    • S. Orion var. 15+
    • Salmonella

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